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. 2020 Jul 24;21(15):5256. doi: 10.3390/ijms21155256

Figure 5.

Figure 5

Effect of CAC secretome on ECFC apoptosis. ECFC were treated (n:3 per condition) with EBM-2 full (5% FBS + Growth factors, Full); serum free EBM-2 medium as basal condition (B), 50 or 100 ng/μL secretome of un-stimulated, control CAC (C50 and C100 respectively) and 50 or 100 ng/μL secretome of CAC pre-incubated whit AP factors (AP50 and AP100) for 24 h. Flow cytometry analysis was done using Annexin-V and Propidium Iodide. Graphs indicate the percentage of (A) representative dot-plots with propidium iodide (PE) and annexin-V (PB450 labelling) is shown for all conditions tested. (B) Early or (C) late apoptotic cells seen, represented as mean ± SE.