Figure 1.

Experimental outline of the comparison of size-exclusion column-based (SEC) versus density-based small extracellular vesicle (sEV) isolations. Supernatant of dissociated lymphatic tissues was separated by differential ultracentrifugation and the resulting 100 K pellet was resuspended and split into two equal parts for direct method comparison. Equal volumes were loaded on either SEC columns or on a sucrose density cushion. Resulting sEV fractions were compared for yield, purity and functionality.