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The central composite design showing the independent variables and levels used in the experiments.
| Factors | Codes | Variable Levels | ||||
|---|---|---|---|---|---|---|
| 2 | −1 | 0 | −1 | −2 | ||
| DPPC (µmol) | X1 | 1 | 2 | 3 | 4 | 5 |
| DDAB (µmol) | X2 | 1 | 1.5 | 2 | 2.5 | 3 |
| CH (µmol) | X3 | 1 | 1.5 | 2 | 2.5 | 3 |
| CMNPs (mg/mL) | X4 | 0.1 | 0.35 | 0.6 | 0.85 | 1.1 |
