Figure 5.

Expression of ALDH activity in culture. Cells were extracted from muscle biopsies of human donors of different ages and grown in culture in proliferating medium. Seven, 14, and 21 days after onset of cultures, ALDH expression was assessed using ALDEF, and the percentage of myogenic cells assessed using PE‐labelled anti‐CD56 antibody. (A) Increased proportions of cells express ALDEF and CD56 over time, and a few cells remain CD56⁺/ALDEF⁻. This behaviour is observed regardless of the ages of donors (B). The microscopic cytofluorescence observation of cultures following induction of cell differentiation indicates that ALDEF is also oxidized and accumulates in both myoblasts and myotubes (C).