Figure 9.

Expression of isoenzyme mRNAs in skeletal muscle tissue and cells. (A) The expression of isoenzymes was assessed and compared in human muscle (red, n = 2), liver (blue, n = 2), and kidney (yellow, n = 2) biopsies by Q‐PCR, underlining the presence of ALDH1A1, 1B1, 1L1, 2, 3A2, 5A1, 6A1, and 9A1. (B) The expression was analysed in the total population of freshly dissociated cells from human biopsies highlighting the presence of isoenzymes involved in the metabolism of retinoic acid (ALDH1A1, 1A2, 1A3, and 1B1) and of ALDH2, 3A2, 6A1, 7A1, and 9A1. (C) The expression was analysed and compared in primary cell cultures in proliferation and in differentiation (n = 4), underlining the presence of ALDH1A1, 1A3, 1B1, 2, 3A2, 7A1, and 9A1. No significant differences were observed between the proliferation and differentiation stages. Data are presented as mean and SD.