Table 5.
Genes, primer sequences and amplicon sizes.
| Gene | GeneBank Accession Numbers | Sense, Antisense Primer (5′–3′) | Amplicon (bp) |
|---|---|---|---|
| MR | ENSSSCG00000037766 | AGTGTTCTTCAAAAGAGCAGTGG, CCTCGTGGATCCCTTTCAAC | 188 |
| GR | NM_001008481.1 | GTTCCAGAGAACCCCAAGAGTTCA, TCAAAGGTGCTTTGGTCTGTGGTA | 173 |
| CRHR1 | NM_001144110.1 | CTCATCTCAGCCTTCATCCTG, CGAACATCCAGAAGAAGTTGG | 151 |
| CRHR2 | NM_001144118.1 | CAGGGTTTCTTCGTGTCTGTC, GTCTGCTTGATGCTGTGGAAG | 173 |
| TNF-α | NM_214022.1 | TCCTCACTCACACCATCAGC, TAGTCGGGCAGGTTGATCTC | 199 |
| IL-6 | NM_214399.1 | TGCTTCTGGTGATGGCTACTG, TTCTGCCAGTACCTCCTTGC | 209 |
| IL-10 | NM_214041 | AGCCAGCATTAAGTCTGAGAAC, CCTCTCTTGGAGCTTGCTAA | 394 |
| ACTB * | ENSSSCT00000042531 | TCTGGCACCACACCTTCT, TGATCTGGGTCATCTTCTCAC | 114 |
| TBP * | NM_003194.5 | AACAGTTCAGTAGTTATGAGCCAGA, AGATGTTCTCAAACGCTTCG | 153 |
MR (mineralocorticoid receptor; NR3C2), GR (glucocorticoid receptor; NR3C1), CRHR1/CRHR2 (corticotropin releasing hormone receptor 1/2), TNF-α (tumour necrosis factor-alpha; TNFA), IL-6 (interleukin-6; IL6), IL-10 (interleukin-10; IL10), ACTB (actin beta), TBP (TATA-box binding protein); * reference gene.