Table 2.
Computational Merging and Meta-Analytical Pathway Analysis (DIANA mirPATH) of Differentially Expressed Exosomal miRNA in Normotensive Pregnancies.
| Exosomal miRNA Identified | Mean Expression Values (Log2) | Kyoto Encyclopedia of Genes and Genomes Analysis (p ≤ 0.05) | ||||
|---|---|---|---|---|---|---|
| Normotensives (30 ± 1 Weeks) |
Normotensives (38 ± 1 Weeks) |
Computational DIANA miRPath microT-CDS Target Pathway | p Value | Target Genes | Target Physiological Function | |
| hsa-miR-451a | 11.06 ± 0.03 | 9.95 ± 0.01 | Estrogen signaling (hsa04915) | 3.0 × 10−3 | ATF2 | Cell cycle regulation, Proapoptotic protein regulation |
| hsa-miR-302d-3p | 7.54 ± 0.21 | 9.24 ± 0.81 | Estrogen signaling (hsa04915) | 8.9 × 10−5 | ESR1, ATF6B, CREB5, CREB1, SOS1, KCNJ6, SHC4, PIK3CA | Cell cycle regulation, Proapoptotic protein regulation |
| Proteoglycans in cancer (hsa05205) | 1.0 × 10−3 | ESR1, PDCD4, SMAD2, SDC1, PTCH1, ROCK2, RDX, ERBB3, FZD3, ANK2, TFAP4, SOS1, PIK3CA, CD44 | Cell migration and Invasion, Cell growth and survival | |||
| hsa-miR-223-3p | 4.95 ± 0.1 | 5.5 ± 0.03 | Mucin-type O-Glycan biosynthesis (hsa00512) | 2.5 × 10−6 | GALNT7, GALNT18 | Protein secretion, stability, processing and function |
ND = not detected. All values are represented by mean ± SEM. All functional Kyoto Encyclopedia of Genes and Genomes analyses were performed using DIANA miRPATH (V3.0) together with DIANA-microT-CDS 5.0 algorithms (interactions with a p-value ≤ 0.05 were considered).