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| gene | forward primer (5′ → 3′) | reverse primer (5′ → 3′) | amplicon size (bp) | NCBI reference sequence |
|---|---|---|---|---|
| IL-1β | TGAGCACCTTCTTTTCCTTCA | TTGTCTAATGGGAACGTCACAC | 101 | NM_008361.3 |
| IL-6 | TCTAATTCATATCTTCAACCAAGAGG | TGGTCCTTAGCCACTCCTTC | 119 | NM_031168.2 |
| TNF-α | TCTTCTGTCTACTGAACTTCGG | AAGATGATCTGAGTGTGAGGG | 108 | NM_013693.3 |
| β-actin | AAGGCCAACCGTGAAAAGAT | GTGGTACGACCAGAGGCATAC | 109 | NM_007393.5 |
