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. 2020 Jul 24;3(4):759–772. doi: 10.1021/acsptsci.0c00031

Figure 2.

Figure 2

Binding of defined AM(37–52) variants to purified MBP-RAMP-CLR ECD fusion proteins. (A, B) Scatter plot of pKI values from competition FP assays for (A) previously reported, rationally designed AM variants34 or (B) new AM variants incorporating library-identified substitutions. Numbers below the x-axis signify the RAMP1, -2, or -3 complexes. (C) Representative competition binding curves for two key variants compared to wild-type. See Table S1 for pKI values with SEM, selectivity comparisons, and associated statistical analyses.