Phylogenomic analysis at the nucleotide level of phage S144, its two closest relatives, and 93 phage genomes representative for each genus infecting Enterobacteriaceae strains and the genome of the human herpesvirus as outgroup. The tree has been built using VICTOR with the formula d0, recommended for phages, and aesthetically modified with iTOL. Bootstrap values higher than 50% are indicated on the branches. The branch lengths of the resulting VICTOR trees are scaled in terms of the used distance formula. S144 and the other two phages in the Loughboroughvirus virus are highlighted in pink and the representative from the closest genus, the Rosemountvirus genus, in purple. For each phage, we indicated the accession number and the family, according to ICTV.