Table 2.
Open reading frames (ORFs) coding for structural proteins in the genome of phage S144: their putative functions, functional category, assigned cluster; protein molecular weight in KDa, number of unique peptides and sequence coverage. The full annotation table with notes on similarities and homologies found for each predicted ORF in the databases is reported in the Supplementary material. Abbreviations: put. = putative; prot. = protein; hyp. = hypothetical.
| ORF | Product | Functional Category | Cluster | Proteins | |||
|---|---|---|---|---|---|---|---|
| gp | MW (kDa) | Unique Peptides | Coverage (%) | ||||
| ORF03 | put. dihydrofolate reductase | nt biosynthesis | A | gp03 | 25 | 3 | 25 |
| ORF08 | terminase, large subunit | packaging | B | gp08 | 54 | 2 | 4 |
| ORF09 | portal prot. | capsid morphogenesis | B | gp09 | 58 | 12 | 27 |
| ORF10 | scaffold prot. | capsid morphogenesis | B | gp10 | 27 | 2 | 5 |
| ORF11 | major capsid prot. | capsid morphogenesis | B | gp11 | 35 | 14 | 49 |
| ORF12 | put. head-to-tail connector complex 1 | capsid morphogenesis | B | gp12 | 18 | 10 | 49 |
| ORF14 | put. head-to-tail connector complex 3 | capsid morphogenesis | B | gp14 | 14 | 5 | 52 |
| ORF15 | put. head-to-tail connector complex 4 | capsid morphogenesis | B | gp15 | 17 | 3 | 22 |
| ORF16 | put. tail prot. 1 | tail morphogenesis | B | gp16 | 21 | 3 | 24 |
| ORF17 | put. tail prot. 2 | tail morphogenesis | B | gp17 | 111 | 25 | 33 |
| ORF18 | put. tail prot. 3 | tail morphogenesis | B | gp18 | 22 | 17 | 80 |
| ORF20 | put. sheath prot. | tail morphogenesis | B | gp20 | 42 | 9 | 32 |
| ORF21 | put. tube prot. | tail morphogenesis | B | gp21 | 16 | 6 | 26 |
| ORF23 | put. tape measure prot. | tail morphogenesis | B | gp23 | 60 | 10 | 26 |
| ORF24 | conserved hyp. prot. | tail morphogenesis | B | gp24 | 32 | 11 | 31 |
| ORF25 | conserved hyp. prot. | tail morphogenesis | B | gp25 | 14 | 4 | 27 |
| ORF26 | put. baseplate prot. 1 | baseplate morphogenesis | B | gp26 | 35 | 9 | 32 |
| ORF27 | put. puncturing prot. | baseplate morphogenesis | B | gp27 | 23 | 6 | 50 |
| ORF28 | put. baseplate prot. 2 | baseplate morphogenesis | B | gp28 | 14 | 5 | 45 |
| ORF29 | put. baseplate prot. 3 | baseplate morphogenesis | B | gp29 | 42 | 10 | 30 |
| ORF30 | put. baseplate prot. 4 | baseplate morphogenesis | B | gp30 | 24 | 7 | 33 |
| ORF31 | put. tail fiber prot. | tail morphogenesis | B | gp31 | 48 | 9 | 24 |
| ORF35 | put. endolysin | lysis | B | gp35 | 20 | 5 | 28 |
| ORF36 | put. IM-spanin | lysis | B | gp36 | 12 | 5 | 38 |
| ORF37 | put. OM-spanin | lysis | B | gp37 | 10 | 1 | 10 |
| ORF40 | hyp. prot. | hyp. | C | gp40 | |||
| ORF43 | thymidylate synthase | nt biosynthesis | C | gp43 | 33 | 5 | 21 |
| ORF54 | conserved hyp. prot. | hyp. | C | gp54 | 19 | 2 | 21 |
| ORF55 | put. P-loop with nucleoside triphosphatehydrolase | nt biosynthesis | C | gp55 | 33 | 2 | 9 |
| ORF58 | conserved hyp. prot. | hyp. | C | gp58 | 49 | 5 | 16 |
| ORF69 | conserved hyp. prot. | hyp. | D | gp69 | 96 | 2 | 4 |
| ORF79 | conserved hyp. prot. | hyp. | E | gp79 | 21 | 2 | 14 |