Table A5.
Summary of the restriction analysis of phage S144 DNA, propagated on C. sakazakii CS1, S. Infantis S15 and S. Muenster S394. DNA from phage Lambda (methylated and unmethylated) have been used as controls. The restriction site for each of the nine used enzymes is specified and in each cell, it is indicated if the DNA was cut (1, in orange) or not (0, in white). SspI and PacI, the only two enzymes able to cut S144 DNA, are in bold. RE, restriction enzymes; RS, restriction sites, met., methylated; unmet., unmethylated.
| RE | RS | met. Lambda | unmet. Lambda | Expected RS for unmet. Lambda | S144 on C. sakazakii | S144 on S. Muenster | S144 on S. Infantis | Expected RS for S144 |
|---|---|---|---|---|---|---|---|---|
| XhoI | C↓TCGAG | 1 | 1 | 1 | 0 | 0 | 0 | 2 |
| GAGCT↑C | ||||||||
| EheI | GGC↓GCC | 1 | 1 | 1 | 0 | 0 | 0 | 5 |
| CCG↑CGG | ||||||||
| SspI | AAT↓ATT | 1 | 1 | 20 | 1 | 1 | 1 | 6 |
| TTA↑TAA | ||||||||
| Bsp120I | G↓GGCCC | 1 | 1 | 1 | 0 | 0 | 0 | 3 |
| CCCGG↑G | ||||||||
| Cfr42I | CCGC↓GG | 1 | 1 | 4 | 0 | 0 | 0 | 3 |
| GG↑CGCC | ||||||||
| SspDI | G↓GCGCC | 1 | 1 | 1 | 0 | 0 | 0 | 5 |
| CCGCG↑G | ||||||||
| MauBI | CG↓CGCGCG | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
| GCGCGC↑GC | ||||||||
| PacI | TTAAT↓TAA | 0 | 1 | 0 | 1 | 1 | 1 | 1 |
| AAT↑TAATT | ||||||||
| NotI | GC↓GGCCGC | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
| CGCCGG↑CG |