Table 2.
Statistical analysis of apoptosis assay results (mean ± SD (%)).
| Cell Line | PAM/TRAIL | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Vehicle | TRAIL | zVAD | DR5/Fc | NAC | PAM | Vehicle | zVAD | DR5/Fc | NAC | |
| HeLa | 7.0 ± 0.5 | 7.7 ± 0.3 | 8.2 ± 1.3 | 8.3 ± 1.3 | 8.2 ± 0.9 | 21.1 ± 1.4 * | 64.3 ± 1.6 *** | 27.4 ± 1.2 * | 19.2 ± 1.1 | 27.2 ± 2.0 * |
| A549 | 9.6 ± 0.2 | 14.9 ± 0.5 | 11.6 ± 0.6 | 12.0 ± 0.4 | 11.6 ± 1.1 | 24.6 ± 0.4 * | 61.3 ± 2.1 *** | 31.7 ± 1.4 * | 15.6 ± 0.6 | 29.4 ± 1.4 * |
HeLa and A549 cells were pretreated with 25 µM of zVAD, 20 ng/mL DR/Fc or 5 mM NAC for 1 h before TRAIL treatment in the absence or presence of PAM. After 24 h incubation, cell death was determined by FACS analysis following Annexin V and propidium iodide staining. DMSO was used as a vehicle. Statistical analysis was performed using a one-way ANOVA test followed by Dunnett’s test for comparisons. * and *** indicate significant differences from the control group (* p < 0.05, *** p < 0.001).