Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 23;21(15):5217. doi: 10.3390/ijms21155217

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Validation and characterization of circRNAs. (A) Relative circRNA expression measured by qRT-PCR. circRNA expression was calculated as a fold change of AA over HA follicles. circRNA expression in HA was set to 1 for up-regulated genes and −1 for down-regulated genes. (B) Representative examples of PCR products purified and sequenced to confirm circRNA junction sites by Sanger sequencing, n = 3. (C) qRT-PCR for the abundance of circRNAs (n = 6) and mRNAs (n = 6) treated with RNase R. The amount of circRNAs and mRNAs was calculated as fold change of RNase R over controls. The expression values in the controls were set to 1 for up-regulated genes and −1 for down-regulated genes. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Follicles were isolated in batches at three different time points, each batch containing 15–20 ovaries.