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. 2020 Aug 4;21(15):5581. doi: 10.3390/ijms21155581

Figure 4.

Figure 4

AMPK Activation Reinforces the Cortical Actin Network and Counters Stress-Fiber Formation During LPS Challenge. HMECs were transfected with control non-targeting siRNA or α1AMPK siRNA (50 nM) for 48 h and treated with DMSO or 991 (1μM) for one hour. They were then exposed to LPS (50 μM) for six hours. After fixation, HMECs were stained with Alexa Fluor 568 Phalloidin. (a) Representative fluorescence microscopy images of F-actin. Arrows indicate peripherical actin; arrowheads indicate centralized stress fibers; (b) Quantification of transcytoplasmic actin filaments; (c) Quantification of global F-actin amounts, which were assessed by integrated density measurement. Nuclei are stained with DAPI. Scale bar, 25 μm. Data are expressed as means ± SD (three biological replicates for each condition). # p < 0.05 is relative to corresponding untreated HMECs, $ p < 0.05 is relative to corresponding LPS-treated HMECs, and * p < 0.05 is relative to cells transfected by scrambled siRNA. The data underwent two-way ANOVA.