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. 2020 Jul 31;21(15):5470. doi: 10.3390/ijms21155470

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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LC-ESI MS and high-performance liquid chromatography (HPLC) analysis of the glutathione (GSH) modification by VAS3947. (A,B) Extracted ion current chromatograms and corresponding mass spectra are shown for VAS3947, VAS3947/GSH mixtures at indicated concentrations (20 min incubation), and the cell extracts pretreated with 4 µM VAS3947. (C) HPLC analysis for VAS3947, the oxazole-2-thiol, and VAS3947/GSH mixtures at various concentrations. (D) MS and HPLC analyses indicate that the thiol function of GSH is alkylated by the benzyltriazolopyrimidine moiety of VAS3947, with the oxazole-2-thiol moiety of VAS3947 serving as a leaving group.