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. 2020 Jul 31;21(15):5470. doi: 10.3390/ijms21155470

Figure 6.

Figure 6

Effect of VAS3947 on cytoplasmic and mitochondrial reactive oxygen species (ROS) production in MV-4-11 cell line. Effect of VAS3947 (4 μM) on (A) cytoplasmic ROS production measured by 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) fluorescence; (B) mitochondrial ROS production measured by MitoSOX fluorescence; (C) mitochondrial membrane potential measured by tetramethyl-rhodamine ethyl ester (TMRE) fluorescence; and (D) mitochondrial biomass measured by MitoTracker fluorescence. (E) Effect of VAS3947 (4 μM) and NEM (8 μM) on cytoplasmic ROS production measured by CM-H2DCFDA fluorescence. ROS measurement at 0 h (A–D) and control (E) was determined and established at 1. Data are shown as normalized fluorescence with respect to the control. Data are shown as mean ± SEM of n = 3 independent experiments. Student’s t-test was performed comparing VAS3947 conditions to control (* p < 0.05; ** p < 0.01; *** p < 0.001).