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. 2020 Jul 31;21(15):5470. doi: 10.3390/ijms21155470

Figure 7.

Figure 7

Effect of VAS3947 on unfolded protein response (UPR) proteins and apoptosis in MV-4-11 cell lines. (A,B) Western blot analysis showing the effect of VAS3947 (4 µM) on the expression of UPR proteins and their phosphorylation status at different indicated time points. (A) The activation of the PKR-like endoplasmic reticulum kinase (PERK) pathway is shown by the phosphorylation of eukaryotic initiation factor 2 alpha (eIF2α). (B) The activation of inositol-requiring enzyme 1α (IRE1α) is shown by the phosphorylation of c-Jun N-terminal protein kinase 1 (JNK1) and P38MAPK (mitogen-activated protein kinase). (C) Western blot analysis showing the activation of apoptosis through caspase 9 and PARP proteins’ cleavage at different indicated time points after VAS3947 (4 µM) treatment. The β-actin was used as loading control.