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. 2020 Jul 31;21(15):5470. doi: 10.3390/ijms21155470

Figure 8.

Figure 8

Glutathione (GSH) or N-acetyl cysteine (NAC) supplementation prevents of MV-4-11 cells from VAS3947-induced apoptosis. (A) MV-4-11 cells were preincubated with GSH (1 mM) for 2 h, washed, and incubated with VAS3947 prior annexin V-positive cell detection by flow cytometry at the indicated timepoints. Data are shown as mean ± SEM of n = 3 independent experiments. (*** p < 0.001) (B) GSH production measured over time in MV-4-11 cells incubated with NAC (1 mM). Data are shown as mean ± SEM of n = 3 independent experiments. (C) Effect of VAS3947 (4 µM) on intracellular GSH level in the presence or absence of a 2 hour-NAC preincubation in MV-4-11 cells. Data are shown as mean ± SEM of n = 3 independent experiments. Student’s t-test was performed to compare various conditions to their corresponding control counterparts (* p < 0.05). (D) Effect of NAC (1 mM) supplementation on VAS3947 (4 µM)-induced apoptosis in MV-4-11 cells. Student’s t-test was performed for each condition, comparing them to their corresponding control counterparts (** p < 0.01; *** p < 0.001). (E) Western blot analysis of apoptotic protein expressions in MV-4-11 cells after treatment as depicted in Figure 8D.