Table 2.
Sequences and physicochemical properties of the synthesized StAMP-1-11 peptides. StAMP-1 corresponds to the C-terminal amidated first lead peptide sequence 18–27.
| Peptide | Sequence 1 | Monoisotopic Mass (Da) | Net Charge | Boman Index (kcal/mol) | Hydro-Phobic Ratio (%) | Rt 3 | |
|---|---|---|---|---|---|---|---|
| Theoretical | Measured 2 | ||||||
| StAMP-1 | GKKPGGWKAK-NH2 | 1054.64 | 1054.64 | +5 | 1.52 | 20 | 0.40 |
| StAMP-2 | GKKWGGWKAK-NH2 | 1143.67 | 1143.67 | +5 | 1.29 | 30 | 1.75 |
| StAMP-3 | GKKPWGWKAK-NH2 | 1183.70 | 1183.70 | +5 | 1.38 | 30 | 2.17 |
| StAMP-4 | GKKPGWWKAK-NH2 | 1183.70 | 1183.70 | +5 | 1.38 | 30 | 2.05 |
| StAMP-5 | GKKWWGWKAK-NH2 | 1272.72 | 1272.72 | +5 | 1.15 | 40 | 5.21 |
| StAMP-6 | GKKWGWWKAK-NH2 | 1272.72 | 1272.72 | +5 | 1.15 | 40 | 5.39 |
| StAMP-7 | GKKPWWWKAK-NH2 | 1312.76 | 1312.76 | +5 | 1.24 | 40 | 5.70 |
| StAMP-8 | GKKWWWWKAK-NH2 | 1401.78 | 1401.78 | +5 | 1.01 | 50 | 8.77 |
| StAMP-9 | GRRPWWWRAR-NH2 | 1424.78 | 1424.78 | +5 | 4.99 | 40 | 6.65 |
| StAMP-10 | GRRWWWWRAR-NH2 | 1513.81 | 1513.81 | +5 | 4.76 | 50 | 9.20 |
| StAMP-11 | GRRPLLLRAR-NH2 | 1205.79 | 1205.79 | +5 | 4.21 | 40 | 2.54 |
1 Amino acid substitutions are shown in bold, 2 Measured by high-resolution mass spectrometry, 3 Retention time (min) on an analytical RP-HPLC C18-column using a fixed mobile phase gradient.