. 2020 Jul 29;21(15):5381. doi: 10.3390/ijms21155381

Table 2.

Quantitative analysis of miR expression in trauma patients stratified by injury pattern.

		miR-124-3p		miR-338-3p		miR-423-3p
		n	mean ± SD	n	mean ± SD	n	mean ± SD
PT	Ct			1	39.8 ± 0	8	32.4 ± 2.9
	∆Ct				13.6 ± 0		4.7 ± 1.2
	∆∆Ct				13.6 ± 0		2.6 ± 1.2
	2^−∆∆Ct				0.0		0.2 ± 0.2
PT + TBI	Ct	7	37.0 ± 3.0	3	38.2 ± 1.3	13	31.3 ± 3.5
	∆Ct		11.8 ± 3.8		13.5 ± 1.9		3.9 ± 1.3
	∆∆Ct		11.8 ± 3.8		13.5 ± 1.9		1.8 ± 1.3
	2^−∆∆Ct		0.0		0.0		0.4 ± 0.2
isTBI	Ct	4	34.7 ± 2.7	5	38.3 ± 1.6	12	30.7 ± 1.4
	∆Ct		7.5 ± 1.3		10.4 ± 2.0		2.4 ± 1.5
	∆∆Ct		7.5 ± 1.3		10.4 ± 2.0		0.3 ± 1.5
	2^−∆∆Ct		0.0		0.0		1.5 ± 2.3

Abbreviations: PT = polytrauma, isTBI = isolated traumatic brain injury, SD = standard deviation, Ct = cycle threshold. Quantitative analysis of target miRNA delta-delta Ct method (2^−∆∆Ct): after normalization of Ct values of the target genes (all samples, n = 38) against the housekeeping gene (∆Ct_Target = Ct_SNORD95 − Ct_Target), the ∆∆Ct value was calculated using healthy volunteers as the control cohort (∆∆Ct_Target = ∆Ct_[V] − ∆Ct_Target).