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. 2020 Jul 30;21(15):5459. doi: 10.3390/ijms21155459

Figure 3.

Figure 3

TML-6 suppresses Aβ production in the N2a/APPswe stable cell line. (A) The N2a cell line showed stable over-expression of APP with wild-type full-length (N2a/APP) and Swedish mutant (N2a/APPswe) cells by Western blotting. The N2a/APPswe stable cell line was used to examine Aβ 40 and Aβ42 levels in culture medium in response to traditional curcumin and TML-6 by ELISA test. N2a/APPswe stable cells were treated with different doses of traditional curcumin (B) and TML-6 (C) as indicated in the figures. The cell morphological change or viability in each treatment was captured and represented for comparison at the lower panel, as indicated. After 24 h incubation, the culture medium was examined for levels of Aβ40 and Aβ42 by LEGEND MAX™ β-Amyloid X-40 and X-42 ELISA Kit. The significant differences between the treated and control cells are represented as the mean with a standard deviation (SD) error bar, and p value < 0.05 was considered significant (* p < 0.05, ** p < 0.01, *** p < 0.001). The cell density, viability, and morphological changes by micrographs were similar between curcumin and TML-6, probably due to cytotoxicity at the highest doses of curcumin (20 μg/mL) and TML-6 (4.19 μg/mL) in N2a/APPswe stable cells.