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. 2020 Aug 3;21(15):5547. doi: 10.3390/ijms21155547

Figure 1.

Figure 1

Characterization of the atmin7 mutant. (a). Phylogenetic analysis of the ARF-GFF protein family in Arabidopsis. (b). Schematic representation of the T-DNA insertion site in atmin7 mutant. Heavy black lines and gray rectangles indicate introns and exons, respectively. The T-DNA insertion site for Salk_013761 is shown. The position and orientation of primers used for qRT-PCR are indicated. (c). Relative AtMIN7 transcript abundance in the WT, atmin7, and 35S::MIN7-GFP plants. The experiment was repeated three times with similar results and the mean +/- SD for the combined data from three replicates are shown. Unpaired two-tailed Student’s t-tests with WT plants indicated that the transcript level of AtMIN7 was significantly reduced in atmin7 but not 35S::MIN7-GFP (* p < 0.01). (d). Morphological phenotype did not differ between vegetative rosettes of four-week-old WT, atmin7, and 35S::MIN7-GFP plants.