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Confirmation that all germinated spores carry pHT315-PgerR-gerRB-SGFP2. Colony PCR was carried out on 24 independent colonies from individual spores of B. cereus 003 carrying pHT315-PgerR-gerRB-SGFP2, as described in Methods. Lanes are: M, GeneRuler 1 kb DNA ladder (Thermo Fisher Scientific); 1–24, PCR from B. cereus 003 carrying pHT315-PgerR-gerRB-SGFP2. The black arrow indicates the DNA fragment of PgerR- gerRB-SGFP2.
