Figure 1.

Single-cell tracking of hMSCs on nanopatterns of low (S₁₈) and high (S₉₀) local surface adhesiveness under chondrogenic conditions for 40 h. (a) Selected time-lapse bright-field images superimposed with fluorescence live images of cell nuclei (Hoechst 33342 nuclear stain). Scale bar = 500 μm. (b) Representative migratory trajectories for individual cells (n = 30). The beginning of each trajectory has been tied to the origin of the Cartesian coordinate system. Trajectories of different cells are shown in different colors. (c) Top: Quantification of the Euclidean distance covered by individual cells. Red dashed line indicates the evolution in the 99th percentile with the increasing local surface adhesiveness (n = 3000). Bottom: Quantification of mean link displacement of individual cell trajectories (n = 30). The blue dashed line indicates the evolution of the mean with the increasing local surface adhesiveness. Results are given as the mean ± SE.