Figure 5.

Resveratrol promoted L. paracasei ATCC334 aggregation. (A) Representative micrographs of live L. paracasei treated for 1 h 30 with increasing doses of Resveratrol from 5 µM (Resv 5) to 50 µM (Resv 50) and observed in phase contrast microscopy. In all images, the white scale bar = 10 μm. For each condition, the percentage of bacteria ± SEM forming aggregates is indicated in graph (B) and the mean number of bacteria per aggregate ± SEM is shown in graph (C). At least, 200 bacteria were counted per condition. * p < 0.05, ** p < 0.01 (versus untreated). (D) Sedimentation assay. Optical density at 600 nm (OD₆₀₀) was measured on a static culture of L. paracasei untreated or treated with increasing doses of Resveratrol from 5 µM (Resv 5) to 50 µM (Resv 50), after 1 h 30 at 37 °C. Results are expressed as mean optical density at 600 nm ± SEM of at least three separate experiments. ** p < 0.01 (versus untreated). (E) Schematic representation of the effect of Resveratrol treatment on aggregation, adhesion and biofilm formation by L. paracasei.