Figure A4.

Combination of Resveratrol and L. paracasei did not elicited an exacerbated pro-inflammatory response. (A) J774 macrophages were untreated or treated with biofilm-grown living L. paracasei at a MOI of 40 for 4 h. L. paracasei were grown under biofilm conditions in the absence (BF) or presence of increasing doses of Resveratrol, from 5 µM (BF Resv 5) to 50 µM (BF Resv 50). TNF-α secretion (pg/mL) was determined by ELISA. Results are expressed as mean ± SEM of at least three independent experiments. (B) J774 macrophages were treated as in A but LPS treatment (100 ng/mL) was added concomitantly to L. paracasei treatment.