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. 2020 Jul 30;21(15):5425. doi: 10.3390/ijms21155425

Figure 1.

Figure 1

Promoter methylation levels of LMX1A in non-small cell lung cancer (NSCLC). (A) DNA methylation array data for LMX1A in 41 tissue samples from healthy individuals, 458 tissue samples from lung adenocarcinoma (LUAD) patients, and 364 tissue samples from lung squamous cell carcinoma (LUSC) patients from the Smart App (http://www.bioinfo-zs.com/smartapp) are shown. The results are shown as average (AVG) β values for the probes. Black lines indicate the mean AVG β value. The p-values for LMX1A methylation levels among the groups (normal versus tumor) were determined by the Mann–Whitney U test. (B) The promoter methylation statuses of LMX1A in eight NSCLC cell lines were analyzed by methylation-specific PCR (MSP) with methylated and unmethylated-specific primers. P: Positive control; N: Negative control. (C) Gene expression levels of LMX1A and the internal reference GAPDH in eight NSCLC cell lines were analyzed by reverse transcription polymerase chain reaction (RT-PCR). Mr: Molecular marker, or DNA ladder.