Figure 2.

Bisulfite genomic sequencing analysis of the CpG sites located at the promoter region of LMX1A. The LMX1A methylation status in four NSCLC cell lines was analyzed by bisulfite genomic sequencing. Each clone is represented by a row, and 32 CpG sites are represented as circles. Black circles and white circles represent methylated and unmethylated CpG sites, respectively. Arrows indicate the locations of the BS primers, MSP primers, and Q-MSP. TSS: Transcriptional start site; TLS: Translational start site; BS: Bisulfate sequencing; MSP: Methylation-specific PCR; Q-MSP: quantitative methylation-specific PCR.