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. 2020 Jul 30;21(15):5425. doi: 10.3390/ijms21155425

Figure 3.

Figure 3

Correlation between methylation status and gene expression level of LMX1A in NSCLC cell lines. (A) Quantitative DNA methylation levels of LMX1A in eight NSCLC cell lines were analyzed by Q-MSP. Gene expression levels of LMX1A and the internal reference GAPDH were analyzed by quantitative RT-PCR. (B) Quantitative DNA methylation levels of LMX1A in CL1-0, H23, and H1299 cells treated with 5 µM 5-aza-2ʹ-deoxycytidine (DAC) or left untreated were determined by Q-MSP. The results are represented as differences in the methylation index (MI). Gene expression levels of LMX1A and the internal reference GAPDH in CL1-0, H23, and H1299 cells treated with 5 µM DAC or left untreated were analyzed by quantitative RT-PCR. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 (Student’s t-test).