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NS3-encoding DNA transfection of bovine endothelial cell lines. BBrMEC, BLuMEC, BSkMEC, and BUcEC were transfected with the expression vector encoding GFP-tagged BTV8-NS3. Intracellular localization of Hoechst-stained nuclei and GFP-tagged NS3 fluorescence was visualized by fluorescence microscopy (×63 magnification) in blue and green, respectively. Scale bars represent 20 μm.
