Table 2.
Evaluation of specific RNA marker expression by RT-qPCR in bovine endothelial cell lines and bovine aortic primary endothelial cells (BAEC).
| Markers | Normalized Relative Expression | BAEC | BBrMEC | BIntMEC | BLuMEC | BMLNMEC | BOvMEC | BSkMEC | BUcEC |
|---|---|---|---|---|---|---|---|---|---|
| Specific markers for mature endothelial cells | |||||||||
| CD31-PECAM1 | mean | 2641.39 | 0.14 | 0.26 | 0.04 | 0.04 | 0.03 | 2.41 | 0.40 |
| SD | 770.92 | 0.06 | 0.11 | 0.01 | 0.001 | 0.003 | 0.06 | 0.04 | |
| CD143-ACE | mean | 23.42 | 2.23 | 0.74 | 2.46 | 0.75 | 0.05 | 0.24 | 0.19 |
| SD | 8.35 | 1.55 | 0.32 | 0.03 | 0.29 | 0.01 | 0.06 | 0.07 | |
| CD146-MCAM | mean | 5.55 | 1.61 | 0.81 | 2.24 | 6.77 | 0.66 | 0.07 | 0.16 |
| SD | 0.44 | 0.57 | 0.51 | 0.20 | 1.63 | 0.04 | 0.01 | 0.01 | |
| VEGFA | mean | 0.08 | 0.79 | 3.70 | 1.58 | 1.05 | 1.18 | 0.13 | 0.68 |
| SD | 0.01 | 0.27 | 1.79 | 0.16 | 0.35 | 0.06 | 0.01 | 0.04 | |
| VEGFR-1-Flt1 | mean | 0.002 | 0.86 | 0.09 | 0.81 | 1.17 | 0.03 | 1.42 | 0.47 |
| SD | 0.0009 | 0.18 | 0.07 | 0.09 | 0.25 | 0.003 | 0.02 | 0.01 | |
| VEGFR-2-KDR-CD309 | mean | 163.16 | 14.32 | 0.04 | 0.11 | 0.92 | 0.01 | 0.01 | 0.65 |
| SD | 45.71 | 2.33 | 0.03 | 0.04 | 0.24 | 0.01 | 0.003 | 0.09 | |
| vWF | mean | 33.09 | 1.39 | 2.08 | 0.13 | 0.21 | 0.12 | 0.06 | 0.22 |
| SD | 26.91 | 1.67 | 1.11 | 0.03 | 0.06 | 0.11 | 0.01 | 0.07 | |
| Specific markers for lymphatic endothelial cells | |||||||||
| LYVE-1 | mean | 0.002 | 36.40 | 37.31 | 495.99 | 2.55 | 2 461.72 | 1.92 | 1.08 |
| SD | 0.001 | 32.36 | 23.17 | 36.94 | 0.51 | 547.28 | 0.17 | 0.12 | |
| Specific markers for progenitor endothelial cells | |||||||||
| CD117-KIT | mean | 0.04 | 19.08 | 0.0002 | 0.01 | 0.001 | 0.0003 | 0.0003 | 0.0001 |
| SD | 0.03 | 6.32 | 0.00004 | 0.01 | 0.0001 | 0.0001 | 0.0001 | ¤ | |
| CD133-PROM1 | mean | 0.01 | 1.72 | 2.13 | 0.12 | 4.18 | 0.41 | 0.71 | 0.82 |
| SD | 0.001 | 0.92 | 1.29 | 0.01 | 1.19 | 0.02 | 0.08 | 0.09 | |
| CXCR4-CD184 | mean | 172.99 | 1.37 | 1.97 | 0.40 | 1.49 | 0.02 | 0.18 | 2.23 |
| SD | 121.75 | 0.86 | 0.22 | 0.01 | 0.08 | 0.004 | 0.01 | 0.28 | |
| Specific marker for non-endothelial cells (hematopoietic cells) | |||||||||
| CD38 | mean | 0.008 | 0.22 | 4.60 | 0.05 | 0.03 | 0.01 | 0.66 | 0.02 |
| SD | 0.006 | 0.12 | 4.57 | 0.01 | 0.01 | 0.01 | 0.05 | 0.0004 | |
SD: standard deviation; PECAM1: Platelet endothelial cell adhesion molecule; ACE: angiotensin-converting enzyme; MCAM: melanoma cell adhesion molecule; VEGFA: vascular endothelial growth factor A; VEGFR: vascular endothelial growth factor receptor; KDR: Kinase insert domain receptor; LYVE-1: endothelial hyaluronan receptor 1; KIT: proto-oncogene c-Kit; PROM1: prominin-1; CXCR4: Chemokine receptor type 4.