Table 3.
Detection of specific cell markers in bovine endothelial cell lines by flow cytometry.
| Relative Fluorescence Intensity (Arbitrary Units) | BBrMEC | BIntMEC | BLuMEC | BMLNMEC | BOvMEC | BSkMEC | BUcEC | BAEC | HEPC-CB1 | HSkMEC |
|---|---|---|---|---|---|---|---|---|---|---|
| Specific markers for mature endothelial cells | ||||||||||
| CD31 (bovine) | <1 | <1 | <1 | <1 | <1 | <1 | <1 | 4 | <1 | <1 |
| CD143/ACE | 20 | 9 | 25 | 25 | 30 | 17 | 10 | 1 | 13 | 14 |
| CD146/MCAM | 44 | <1 | 291 | 278 | 172 | <1 | 2 | 1006 | 540 | 265 |
| CD105/endoglin | nt | nt | <1 | nt | nt | 3 | 1 | 5 | 190 | 358 |
| eNOS | nt | nt | 14 | nt | nt | 7 | 7 | 451 | nt | 108 |
| Specific markers for progenitor endothelial cells | ||||||||||
| CD133 | 3 | 4 | <1 | 7 | 2 | 2 | <1 | 1 | 190 | 1 |
| CD271 | 5 | 5 | <1 | < 1 | 3 | 20 | <1 | 5 | 525 | 4 |
| CXCR4/CD184 | 14 | 29 | 13 | 27 | 12 | 8 | 14 | 9 | 154 | 12 |
| Specific markers for non-endothelial cells (hematopoietic cells) | ||||||||||
| CD34 | nt | nt | 10 | nt | nt | 3 | 3 | 3 | 2 | 2 |
| CD45 | nt | nt | 3 | nt | nt | 2 | 2 | 3 | 2 | 2 |
nt: non tested; eNOS: endothelial nitric oxide synthase.