Table 5.
Bovine genes and associated primers used for the cell lines characterization by RT-qPCR.
| Primer Names | Associated Genes | Forward Primer Sequence | Reverse Primer Sequence |
|---|---|---|---|
| gusb | Beta-glucuronidase | CTGGTTACTACTTCAAGACG | CTGCTTCATAGTTGGTGTTG |
| hprt1 | Hypoxanthine-guanine phosphoribosyltransferase | ATCCATTCCTATGACTGTGG | ACTTTTATGTCGCCTGTTG |
| ppia | Peptidylprolyl isomerase A | AAGACTGAGTGGTTGGATG | GTCAGCAATGGTGATCTTC |
| cd31 | Platelet endothelial cell adhesion molecule | GAAGACATTATCGGATGCC | TTAATGGCTTCATTGCATGG |
| cd143 | Angiotensin-converting enzyme | GAAATGAAACCCACTTTGAC | TCACGAAGTACCTGATATACG |
| cd146 | melanoma cell adhesion molecule | CTGGTTTTCTGTCCACAAG | CAGAGTAGTCCCTTTGTCC |
| vegfa | Vascular endothelial growth factor A | GCTGTAATGACGAAAGTCTG | GGAAGCTCATCTCTCCTATG |
| vegfr1 | Vascular endothelial growth factor receptor 1 | CAACCACAAAATACAGCAAG | GTGACTCTCTCGATAAACAG |
| vegfr2 | Vascular endothelial growth factor receptor 2 | TGATGAGGAATTTTGTAGGC | ATGGTCTGGTACATTTCTGG |
| vwf | von Willebrand Factor | CAGACACTTCAACAAGACC | TTCCTTGAGTCCTGAAGTC |
| lyve-1 | Lymphatic vessel endothelial hyaluronan receptor 1 | TACTGCCACAACTCATCTG | GTTGAATAAGGGATCATCGG |
| cd117 | proto-oncogene c-Kit | TAGTTCCGTGGACTCTATG | GATGCCAGCTATTCTTCTTC |
| cd133 | prominin-1 | CGACAGAAGAAAAGTGGTC | CGATGCTTATGAACACACAG |
| cxcr4 | chemokine receptor type 4 | GATCCGTATATTCACTTCCG | AAGATGATGGAGTAGACAGTG |
| cd38 | cyclic ADP ribose hydrolase | TTCATGAGTGCCTTCATTTC | TTTTCGCGTATTCATGAGC |