Table 6.
Antibodies used for cell line characterization by flow cytometry.
| Primary Antibody | Corresponding Isotype | Secondary Antibody | |||
|---|---|---|---|---|---|
| Target | Reference and Provider | Isotype | Reference and Provider | Antibody | Reference and Provider |
| CD31 (bovine cross reactivity) |
MA3100, Invitrogen (Cergy Pontoise, France) |
Mouse IgG (serum) | I5381, Sigma-Aldrich (Saint Quentin Fallavier, France) |
Goat anti-mouse IgG-PE | Sc-3738, Santa Cruz (Heidelberg, Germany) |
| CD31(ovine) | MCA1097GA, Bio-Rad (Marnes-la-Coquette, France) |
Mouse IgG (serum) | I5381, Sigma-Aldrich (Saint Quentin Fallavier, France) |
Goat anti-mouse IgG-PE | Sc-3738, Santa Cruz (Heidelberg, Germany) |
| Mouse IgG2a | MCA1210 Bio-Rad (Marnes-la-Coquette, France) |
||||
| CD31-APC | 130-110-808, Miltenyi Biotec (Paris, France) |
Mouse IgG1-APC | 555751, BD Biosciences (Le Pont de Claix, France) |
N.A. | N.A. |
| CD143-PE | FAB929P, R&D Systems (Minneapolis, MN, USA) |
Mouse IgG1-PE | IC002P, R&D Systems (Minneapolis, MN, USA) | N.A. | N.A. |
| CD146-PE | FAB932P, R&D Systems (Minneapolis, MN, USA) |
Mouse IgG1-PE | IC002P, R&D Systems (Minneapolis, MN, USA) | N.A. | N.A. |
| CD133-PE | 130-090-853, Miltenyi Biotec (Paris, France) |
Mouse IgG2b-PE | 130-092-215, Miltenyi Biotec (Paris, France) | N.A. | N.A. |
| CD271-FITC | 130-091-917, Miltenyi Biotec (Paris, France) |
Mouse IgG1-FITC | 130-092-213, Miltenyi Biotec (Paris, France) | N.A. | N.A. |
| CXCR4-PE | FAB170P, R&D Systems (Minneapolis, MN, USA) |
Mouse IgG2a-PE | IC003P, R&D Systems (Minneapolis, MN, USA) | N.A. | N.A. |
| CD34-FITC | 130-081-001, Miltenyi Biotec (Paris, France) |
Mouse IgG2a-FITC | 130-091-837, Miltenyi Biotec (Paris, France) | N.A. | N.A. |
| CD45-FITC | 130-080-202, Miltenyi Biotec (Paris, France) |
Mouse IgG2a-FITC | 130-091-837, Miltenyi Biotec (Paris, France) | N.A. | N.A. |
| CD105-PE | FAB10971P, R&D Systems (UK) | Mouse IgG1-PE | IC002P, R&D Systems (UK) | N.A. | N.A. |
| eNOS | 160880, Cayman chemical (Hamburg, Germany) |
Rabbit IgG (serum) | I5006, Sigma-Aldrich (Saint Quentin Fallavier, France) |
Goat anti-rabbit IgG-PE | Sc-3739, Santa Cruz (Heidelberg, Germany) |
All antibodies target the human proteins except when this is mentioned. N.A.: not applicable.