Figure 5.

(a) Expression of adenylate cyclase 9 (AC9) (green) and β2-adrenergic receptor (red) in healthy substitutes (HS) and psoriatic substitutes (PS) produced with cholera toxin (+CT) or isoproterenol (+ISO). The nuclei were stained with Hoechst (blue). The dotted line indicates the dermo-epidermal junction. Scale bar: 100 μm. (b) Ten micrograms of total protein from skin substitute epidermis were analyzed by immunoblot for the presence of adenylate cyclase 9 (AC9). β-actin was used to control equal loading. (N = 2 donors per condition; n = 2 skin substitutes per donor). One representative immunoblot is shown. (c) Densitometric analyses of the immunoblot from panel (b) (N = 2 donors per condition; n = 2 skin substitutes per donor). Data from healthy or psoriatic substitutes were combined irrespective of treatment regime. The statistical significance was determined using an unpaired t-test (* p-value < 0.05).