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. 2020 Jul 26;21(15):5293. doi: 10.3390/ijms21155293

Figure 3.

Figure 3

Analysis of mast cell chemoattraction and degranulation by different cytokines. (A) The levels of Rantes, SDF1, G-CSF, GM-CSF, MCP1 (top), and IL-8 and SCF (bottom) were measured in the conditioned media (CM) derived from BrCC lines using a multiplex luminex assay (top) and ELISA (bottom). All proteins were at undetectable levels in control media. (B) Analysis of chemoattraction of HMC1 (top) and LAD-2 (bottom) cells using recombinant proteins as chemotactic factor and CM as experimental controls. Low and High refers to the mean of the cytokine concentration found in CM derived from non-aggressive cells and aggressive MDA-MB-231 cells, respectively (specified in the methods section). Cocktail 1 and 2 are the combination of all cytokines at low and high concentrations, respectively. (C) Analysis of degranulation of LAD-2 mast cells in response to recombinant proteins at high concentrations, individually or combined (cocktail 2). Results are shown as mean fluorescence intensity normalized to unstimulated MCs. Data represent the mean ± SEM from four independent experiments by duplicate. * p < 0.05, ** p < 0.01 and *** p < 0.001.