Table 1.
List of primary antibodies used for immunocytochemical and immunoblotting studies. Stemness markers: SSEA-4, stage-specific embryonic antigen-4; Sox2, SRY (sex determining region Y)-box 2; TEX14, testis expressed gene 14; Pmel17, melanocyte protein; IL-18, interleukin-18; ACTH, adrenocorticotropin hormone; α-MSH, alpha melanocyte-stimulating hormone; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
| Antibody | Description | Company | Application | Dilution |
|---|---|---|---|---|
| SSEA-4 | Mouse monoclonal [MC813] | Cell Signaling Technology | IF | 1:500 |
| Sox2 | Rabbit polyclonal | Proteintech | IF | 1:100 |
| WB | 1:2000 | |||
| Connexin 43 | Rabbit polyclonal | Assay biotech | IF | 1:100 |
| WB | 1:500 | |||
| E-cadherin | Mouse monoclonal [SHE78-7] | Calbiochelm | IF | 1:500 |
| Pan-Cytokeratin | Mouse monoclonal [C-11] | GeneTex | IF | 1:200 |
| TEX14 | Rabbit polyclonal | Proteintech | IF | 1:200 |
| WB | 1:1000 | |||
| Pmel17 | Mouse monoclonal [HMB45] | Abcam | IF | 1:200 |
| WB | 1:400 | |||
| ACTH | Rabbit polyclonal | Abcam | IF | 1:100 |
| α-MSH | Rabbit polyclonal | Abcam | IF | 1:100 |
| IL18 | Rabbit polyclonal | Thermo Scientific | IF | 1:50 |
| WB | 1:1000 | |||
| GAPDH | Rabbit polyclonal | Proteintech | WB | 1:8000 |