Reduced donor CD8 T cell Vβ07, 11 and 17 expansion in CVB-BMF mice following allogeneic SPF donor LN cell injection. BM cells were collected from untreated (CON) or LN-cell-infused (BMF) mice from CVB and SPF facilities at day 17 following the infusion of SPF donor LN cells, and were stained with a panel of 15 antibodies specific for T cell receptor β variable regions (Vβ). A, Relative proportions of Vβ subsets in host CD8 T cells from CVB-CON mice and relative proportions of Vβ subsets in donor-type CD8 T cells from CVB-BMF mice. B, Relative proportions of Vβ subsets in host CD8 T cells from SPF-CON mice and relative proportions of Vβ subsets in donor-type CD8 T cells from SPF-BMF mice. C, Proportions of donor-type CD8 T cell Vβ in CVB-BMF and SPF-BMF mice over average proportion of host CD8 T cell Vβ in CVB-CON and SPF-CON mice were calculated as ratios, showing reduced expansion in Vβ 07, Vβ 11 and Vβ 17 CD8 T cells in CVT-BMF mice relative to SPF-BMF mice. Data shown as means with standard errors. *, P<.05; **, P<.01.