FIGURE 6.

Diet‐ and MEF2‐dependent regulation of Cyclin E in muscle enhances stress protection and lifespan (a) Lifespan curve; w¹¹¹⁸; Act88FGS, UAS‐LD‐GFP/UAS‐CycE RNAi; UAS‐MEF2 RNAi/+ (Act88FGS, UAS‐LD‐GFP>UAS‐MEF2 RNAi, UAS‐CycE RNAi) female flies (+/−RU486) fed a high calorie (HC) diet. n = 200 flies. (b) Lifespan curve; w¹¹¹⁸; Act88FGS, UAS‐CycE R/+ (Act88FGS>CycE R RNAi) female flies (+/−RU486) fed a high calorie (HC) diet. n = 200 flies; *p value <.01 (mean lifespan). (c) Mortality plot; genotype described above (+/−RU486). (d) Immunostaining to detect poly‐ubiquitin protein (aggregates, Poly‐Ubiq.) in dissected dorsal longitudinal thoracic muscle from indicated diet; anti–poly‐ubiquitin (green), F‐actin filaments (Phalloidin, Red), and nuclei (DAPI, blue). Genotypes described above, female flies. Red squares ;HC diet. (e) Quantification of large protein aggregates (Poly‐Ubiq.‐positive) from indicated diet and genotypes. Circles represent independent (n = 5–10) dissected dorsal longitudinal thoracic (IFM) muscle segments