FIGURE 5.

Loss of NSD2 downregulates the promoter activities of RB‐associated genes. (a) ChIP‐qPCR of H3K27 acetylation (ac) at promoters of indicated gene loci in Ctr‐ and NSD2‐KD IMR‐90 cells. Data are shown as means ± SD; n = 3. (b) Venn diagram of genes downregulated in NSD2‐KD IMR‐90 cells, RB‐associated genes, and RBL2‐associated genes. (c) qRT‐PCR of representative RB‐associated, cell cycle‐associated genes on day 2 in Ctr‐, NSD2‐, and RB1‐KD IMR‐90 cells. Data are shown as means ± SD; n = 3. (d) Relative expression levels of RB‐ or RBL2‐associated, NSD2‐KD downregulated genes in Ctr‐ and RB1‐KD OIS IMR‐90 cells. Data were obtained from GSE60652. *p < .05, **p < .01, calculated using Student's t test