Extended Data Figure 10. Molecular Maturation Analysis.

a) WGCNA networks generated from annotated primary radial glia, as described in methods, were applied to both primary and organoid radial glia cells. Module eigengenes shown in the heatmap indicate overall higher expression in primary compared to organoid radial glia. b) Pseudoage (x-axis) versus actual age (y-axis) in PFC and V1 radial glia showing PFC are more mature than V1 radial glia. c) Box and whisker plot (min to max, bar at mean) across all cells within a single organoid from all organoids within this study show heterogeneity of maturation is within a single organoid and not between individuals (n=242,349 cells from 37 organoids across 4 independent experiments). d) The parallel pseudoage analysis to the analysis in Fig 3C is shown, but starting with organoid networks for the pseudoage calculation. Graph line shows mean pseduoage score against actual age, shading represents the geometric density standard error of the regression. The same pattern is observable, with organoids failing to recapitulate the molecular maturation of primary radial glia, though genes related to the switch from neurogenesis to gliogenesis are preserved and may account for some of the limited correlation.