Table 1.
Theoretical and measured average masses (mass error < 1 Da) of the Fc/2 and light chain subunit for the bNAb samples from Figs. 2 and 3 (ND not detected)
Subunits | No. of AEBSF adducts | 0 | 1 | 2 | 3 | |
---|---|---|---|---|---|---|
Fc/2 | Theoretical mass (Da) | 23,714.5 | 23,897.5 | 24,080.6 | 24,263.6 | |
Measured mass (Da) | Negative control | 23,713.9 | ND | ND | ND | |
Day-7 positive control | 23,713.6 | 23,896.7 | 24,080.0 | 24,262.9 | ||
Light chain | Theoretical mass (Da) | 23,002.3 | 23,185.3 | 23,368.4 | 23,551.4 | |
Measured mass (Da) | Negative control | 23,002.3 | ND | ND | ND | |
Day-7 positive control | 23,002.1 | 23,185.3 | 23,368.7 | 23,552.3 |