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. 2020 Aug 17;20:257. doi: 10.1186/s12906-020-03052-w

Table 1.

Primer sequences used for real-time PCR analysis

Gene Forward Reverse
h-SREBP2 AAGTCTGGCGTTCTGAGGAA AGGTCCACCTCATTGTCCAC
h-HMGCR CTTGTGTGTCCTTGGTATTAGAGCTT GCTGAGCTGCCAAATTGGA
h-SR-BI CTTAAGAACGTGCGCATCGA TGCTTTTGTGCCTGAACTCC
h-LXRɑ GGAGGTACAACCCTGGGAGT AGCAATGAGCAAGGCAAACT
h-LXRβ CCCCTTCTTCTTCACCCACT CGACTGTGACTGTGACTCCT
h-β-actin CTGGAACGGTGAAGGTGACA AAGGGACTTCCTGTAACAATGCA
r-SREBP2 AGAAGGAGAAAGGCGGACAA TCTCCTGGCGCAGTTTATGA
r-HMGCR AGAGCTGGCTTGAAACACCTGA TTTGGACTGGAGACGGATGTAGA
r-CYP7A1 TTGGAATAAGGAGAAGGAAAGC GGAGTTTGTGATGAAATGGACA
r-miR-33a CCTCATAAGCGGTGCATTGTA TATGCTTGTTCTCGTCTCTGTGTC
r-GAPDH GGTGAAGGTCGGTGTGAACG CTCGCTCCTGGAAGATGGTG

SREBP2 sterol regulatory element binding protein 2; HMGCR HMG-CoA reductase; CYP7A1 cytochrome P450 family 7 subfamily A member 1; LXRα liver X receptor ɑ; LXRβ liver X receptor β; SR-BI scavenger receptor class B type 1; GAPDH glyceraldehyde-3-phosphate dehydrogenase; miR-33a microRNA-33a