Table 2:
Comparison of assembly contiguity and completeness between Arctia plantaginis and 7 publicly available lepidopteran assemblies
| Assembly contiguity | Assembly completeness (%) | |||||||
|---|---|---|---|---|---|---|---|---|
| Assembly size (Mb) | Total scaffolds/contigs | Longest scaffold/contig (Mb) | N50 (kb) | N50 count | Total complete BUSCOs | Single copy BUSCOs | Duplicated BUSCOs | |
| Arctia plantaginis (binned: iArcPla.TrioW, scaffolded assembly) | 585 | 1,069 | 21.5 | 6,730 | 24 | 98.1 | 96.9 | 1.2 |
| Arctia plantaginis (binned: iArcPla.TrioY, scaffolded assembly) | 578 | 1,050 | 24.4 | 9,770 | 18 | 96.4 | 95.3 | 1.1 |
| Arctia plantaginis (binned: iArcPla.TrioW, unscaffolded assembly) | 585 | 1,441 | 11.4 | 2,000 | 75 | 97.4 | 96.4 | 1.0 |
| Arctia plantaginis (binned: iArcPla.TrioY, unscaffolded assembly) | 578 | 1,290 | 23.8 | 4,016 | 37 | 95.1 | 94.1 | 1.0 |
| Arctia plantaginis (unbinned: iArcPla.wtdbg2, unscaffolded assembly) | 615 | 2,948 | 11.3 | 1,840 | 85 | 96.9 | 94.8 | 2.1 |
| Bicyclus anynana | 475 | 10,800 | 5.04 | 638.3 | 194 | 97.6 | 96.8 | 0.8 |
| Bombyx mori | 482 | 696 | 21.5 | 16,796 | 13 | 98.4 | 97.2 | 1.2 |
| Danaus plexippus | 249 | 5,397 | 6.24 | 715.6 | 101 | 98.0 | 96.0 | 2.0 |
| Heliconius melpomene | 275 | 332 | 18.1 | 14,308 | 9 | 97.7 | 96.7 | 1.0 |
| Manduca sexta | 419 | 20,871 | 3.25 | 664.0 | 169 | 96.7 | 93.9 | 2.8 |
| Melitaea cinxia | 390 | 8,261 | 0.668 | 119.3 | 970 | 83.0 | 82.9 | 0.1 |
| Trichoplusia ni | 333 | 1,916 | 8.93 | 4,648 | 27 | 97.4 | 96.6 | 0.8 |
Standard contiguity and BUSCO completeness metrics generated for each genome assembly, highlighting the high-quality A. plantaginis assembly achieved by trio binning. See Fig. 3 for assembly contiguity visualization via cumulative scaffold plots, and Supplementary Table S3 for the full BUSCO analysis summary.