Table 1.
Peculiarities of scDNAseq technologies
| Method | Uniformity | Coverage | Throughput | Suitability for CNA |
|---|---|---|---|---|
| MDA [36–38] | Low | High | Low | N |
| DOP-PCR [17–19] | High | Low | High | Y |
| MALBAC [39] | High | High | Low | Y |
| C-PCR-L [44] | High | Low | High | Y |
| SCI-seq (xSDS) [48] | Medium | Low | High | Y1 |
| DLP [40] | High | Low | High | Y |
| LIANTI [49] | High | High | Low2 | Y |
| TnBC [21] | High | Low | High | Y |
| Flow cytometry [50, 51] | High | Low | High | Y |
| 10x [43] | High | Low | High | Y |
| DLP+ [52] | High | Low | High | Y |
Eleven scDNAseq technologies are listed. Uniformity, coverage, throughput, and suitability for CNA refer to the uniformity of sequencing coverage, the sequencing coverage over the whole genome, the number of cells that can be sequenced at one time, and whether the technology is suitable for CNA calling
1With cell filtering
2High-throughput sequencing can be achieved by adding combinatorial cellular barcodes