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. Author manuscript; available in PMC: 2021 Jun 22.
Published in final edited form as: Dev Cell. 2020 Jun 5;53(6):627–645.e7. doi: 10.1016/j.devcel.2020.05.014

Figure 3. ELs Accumulate within Apoptotic Mitochondria in a Rapid Manner, Coinciding with Mitochondrial BAX Clustering and Cytochrome c Release.

Figure 3.

(A) MCF-7_Cyto c-GFP cells, transfected with RFP-BAX, live imaged every 1 min over 40+ min starting at 4 hrs of STS treatment. Overview images, time-series ROI, YT-coordinate of ROI projected over X. See also Video S5.

(B) Graph of mitochondrial Cyto c-GFP (green traces) and RFP-BAX (red traces) intensities of 4 cells treated and imaged as in (A). Bold traces, mean. Pale traces, individual cells. Dotted traces, reference intensities over time.

(C) MCF-7_GFP-Rab5_RFP-BAX cells, live imaged every 2 min over 40+ min at 4 hrs STS. Images displayed as in (A). See also Video S6.

(D) Graph of mitochondrial GFP-Rab5 (green) and RFP-BAX (red) intensities of 22 cells treated and imaged as in (C). Graph displayed as in (B).

(E) MCF-7_Cyto c-GFP_RFP-Rab5 cells, live imaged every 30 sec over 10+ min at 6 hrs TNFα/AcD. Images displayed as in (A). See also Video S7.

(F) Graph of mitochondrial Cyto c-GFP (green) and Rab5-RFP (red) intensities of 5 cells treated with STS for 4 hrs, and imaged as in (C). Graph displayed as in (B).

Scale bars, 10 μm.