Figure 1. 2D Representation of 3D Data Related to Whole iWAT Depots Reveals Robust UCP1 Upregulation in the iAdFASNKO Mouse.
All panels are light-sheet fluorescence microscopy images of inguinal white adipose tissue (iWAT) processed using the Adipo-Clear technique.
(A and B) 2D representations of 3D reconstruction of adipose sympathetic nerves and thermogenic profile of iWAT from control, iAdFASNKO, and 6°C cold-exposed mice (A). The samples are immunolabeled with anti-TH (green) and anti-UCP1 (orange) and imaged at (A) 1.1× and (B) 24× magnification. Scale bars are provided for each panel.