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. 2020 Aug 11;7:147. doi: 10.3389/fmolb.2020.00147

FIGURE 2.

FIGURE 2

NEAT1 exerted its regulatory role in degenerative HNPC cells through targeting miR-195a. (A) The binding sites between miR-195a and NEAT1 were predicted via the StarBase database. (B) Luciferase reporter assay was used to explore the relationship between NEAT1 and miR-195a. (C,D) Real-time PCR was used to measure the NEAT1 or miR-195a expression in HNPC cells; there were three replicates in each group. (E–G) The expressions of collagen-II, TIMP-1, and MMP-3 were analyzed by Western blot, respectively; there were three replicates in each group. (H,I) Hoechst staining was performed to observe the apoptosis of HNPC cells; there were five replicates in each group. *P < 0.05 vs. control group; #P < 0.05 vs. AGE group; &P < 0.05 vs. AGE + NEAT1 siRNAs group; $P < 0.05 vs. AGE + NEAT1 siRNAs + miR-195a inhibitor group.