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. 2020 Aug 11;7:147. doi: 10.3389/fmolb.2020.00147

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Copyright © 2020 Tang, Dong, Liu and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing of NEAT1 upregulates hsa-miR-195a expression to reverse the BAX/BAK-dependent apoptosis and ECM metabolism in degenerative HNPC cells. (A–C) The expressions of BAX and BAK were analyzed by Western blot, respectively; there were three replicates in each group. (D) Hoechst staining was performed to observe the apoptosis of HNPC cells; there were five replicates in each group. (E,F) The expressions of Collagen-II and MMP-3 were measured by Western blot, respectively; there were three replicates in each group. *P < 0.05 vs. control group; #P < 0.05 vs. AGE group; &P < 0.05 vs. AGE + NEAT1 siRNAs group; $P < 0.05 vs. AGE + NEAT1 siRNAs + miR-195a inhibitor group.